@article{pmid38510150,

title = {Exogenous IL-2 delays memory precursors generation and is essential for enhancing memory cells effector functions},

author = {Shaoying Wang and Margaux Prieux and Simon de Bernard and Maxence Dubois and Daphne Laubreton and Sophia Djebali and Manon Zala and Christophe Arpin and Laurent Genestier and Yann Leverrier and Olivier Gandrillon and Fabien Crauste and Wenzheng Jiang and Jacqueline Marvel},

doi = {10.1016/j.isci.2024.109411},

issn = {2589-0042},

year  = {2024},

date = {2024-04-01},

urldate = {2024-04-01},

journal = {iScience},

volume = {27},

number = {4},

pages = {109411},

abstract = {To investigate the impact of paracrine IL-2 signals on memory precursor (MP) cell differentiation, we activated CD8 T cell  in the presence or absence of exogenous IL-2 (ex-IL-2). We assessed memory differentiation by transferring these cells into virus-infected mice. Both conditions generated CD8 T cells that participate in the ongoing response and gave rise to similar memory cells. Nevertheless, when transferred into a naive host, T cells activated with ex-IL-2 generated a higher frequency of memory cells displaying increased functional memory traits. Single-cell RNA-seq analysis indicated that without ex-IL-2, cells rapidly acquire an MP signature, while in its presence they adopted an effector signature. This was confirmed at the protein level and in a functional assay. Overall, ex-IL-2 delays the transition into MP cells, allowing the acquisition of effector functions that become imprinted in their progeny. These findings may help to optimize the generation of therapeutic T cells.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {P-228 Les modèles mathématiques peuvent prédire l'activité des plaquettes humaines et les expressions protéiques en réponse à diverses stimulations},

author = {Fabrice Cognasse and Kim Anh Nguyen and Marco Heestermans and Charles-Antoine Arthaud and Marie-Ange Eyraud and Amélie Prier and Simon De Bernard and Julien Nourikyan and Anne-Claire Duchez and Stéphane Avril and Olivier Garraud and Hind Hamzeh-Cognasse},

doi = {10.1016/j.tracli.2023.09.272},

year  = {2023},

date = {2023-11-10},

urldate = {2023-11-10},

journal = {Transfusion Clinique et Biologique},

volume = {30},

issue = {S1},

pages = {S147},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid38015712,

title = {Chronological aging impacts abundance, function and microRNA content of extracellular vesicles produced by human epidermal keratinocytes},

author = {Taku Nedachi and Christelle Bonod and Julie Rorteau and Wafae Chinoune and Yuri Ishiuchi and Sandrine Hughes and Benjamin Gillet and Nicolas Bechetoille and Dominique Sigaudo-Roussel and Jérôme Lamartine},

doi = {10.18632/aging.205245},

issn = {1945-4589},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {Aging (Albany NY)},

volume = {15},

number = {22},

pages = {12702--12722},

abstract = {The disturbance of intercellular communication is one of the hallmarks of aging. The goal of this study is to clarify the impact of chronological aging on extracellular vesicles (EVs), a key mode of communication in mammalian tissues. We focused on epidermal keratinocytes, the main cells of the outer protective layer of the skin which is strongly impaired in the skin of elderly. EVs were purified from conditioned medium of primary keratinocytes isolated from infant or aged adult skin. A significant increase of the relative number of EVs released from aged keratinocytes was observed whereas their size distribution was not modified. By small RNA sequencing, we described a specific microRNA (miRNA) signature of aged EVs with an increase abundance of miR-30a, a key regulator of barrier function in human epidermis. EVs from aged keratinocytes were found to be able to reduce the proliferation of young keratinocytes, to impact their organogenesis properties in a reconstructed epidermis model and to slow down the early steps of skin wound healing in mice, three features observed in aged epidermis. This work reveals that intercellular communication mediated by EVs is modulated during aging process in keratinocytes and might be involved in the functional defects observed in aged skin.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}