@article{pmid35184302,

title = {Gene profiling reveals a contact allergy signature in most positive Amerchol L-101 patch test reactions},

author = {Linda Ljungberg Silic and Marine-Alexia Lefevre and Ola Bergendorff and Simon De Bernard and Julien Nourikyan and Laurent Buffat and Audrey Nosbaum and Magnus Bruze and Jean-François Nicolas and Cecilia Svedman and Marc Vocanson},

doi = {10.1111/cod.14077},

issn = {1600-0536},

year  = {2022},

date = {2022-07-01},

urldate = {2022-07-01},

journal = {Contact Dermatitis},

volume = {87},

number = {1},

pages = {40--52},

abstract = {BACKGROUND: Diagnosis of contact allergy (CA) to Amerchol L-101 (AL-101), a marker for lanolin allergy, is problematic. Positive patch test reactions are frequently doubtful or weakly positive and difficult to associate with clinical relevance.



OBJECTIVE: To gain further insight on the allergic or irritant nature of skin reactions induced by AL-101 patch test.



METHODS: We re-tested in a dose-response fashion, 10 subjects with AL-101 CA and performed comprehensive transcriptomic analysis (gene arrays, quantitative real-time polymerase chain reaction [qRT-PCR]) of samples of their skin reactions.



RESULTS: Eight of the 10 CA subjects reacted positively upon re-test, whereas two did not react. Most of AL-101 positive patch tests expressed an allergy signature with strong activation of gene modules associated with adaptive immunity and downregulation of cornification pathway genes. In addition, the breadth of gene modulation correlated with the magnitude of patch test reactions and the concentration of AL-101 applied. However, we observed that some of the positive patch test reactions to AL-101 expressed no/few allergy biomarkers, suggesting the induction of an irritant skin inflammation in these samples.



CONCLUSIONS: This study confirms that AL-101 is an allergen that can cause both contact allergy and contact irritation. Our results also highlight that molecular profiling might help to strengthen clinical diagnosis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid35464449,

title = {Dichotomy in neutralizing antibody induction to  peptide-conjugated vaccine in squalene emulsion contrast with  aluminum hydroxide formulation},

author = {Olivia Bonduelle and Chloé Chaudesaigues and Monica Tolazzi and Ehsan Suleiman and Simon Bernard and Karine Alves and Julien Nourikyan and Mylene Bohec and Laura G Baudrin and Dietmar Katinger and Patrice Debré and Gabriella Scarlatti and Vincent Vieillard and Behazine Combadi`ere},

doi = {10.3389/fimmu.2022.848571},

year  = {2022},

date = {2022-04-01},

urldate = {2022-04-01},

journal = {Front. Immunol.},

volume = {13},

pages = {848571},

publisher = {Frontiers Media SA},

abstract = {W614A-3S peptide is a modified 3S motif of the HIV-gp41 

 (mutation W614A). We previously detected the presence of natural 

 neutralizing antibodies directed against W614A-3S peptide (NAbs) 

 in long-term non-progressor HIV+ patients. Here, we compared the 

 efficacy of W614A-3S peptide formulated in either squalene 

 emulsion (SQE) or in aluminum hydroxide (Alum) in inducing 

 broadly-NAbs (bNAbs). Rabbit and mouse models were used to 

 screen the induction of bNAbs following 4 immunizations. SQE was 

 more efficient than Alum formulation in inducing 

 W614A-3S-specific bNAbs with up to 67%-93% of HIV strains 

 neutralized. We then analyzed the quality of peptide-specific 

 murine B cells by single-cell gene expression by quantitative 

 reverse transcription-PCR and single-cell V(D)J sequencing. We 

 found more frequent germinal center B cells in SQE than in Alum, 

 albeit with a different gene expression profile. The V(D)J 

 sequencing of W614A-3S-specific BCR showed significant 

 differences in BCR sequences and validates the dichotomy between 

 adjuvant formulations. All sixteen BCR sequences which were 

 cloned were specific of peptide. Adjuvant formulations of 

 W614A-3S-peptide-conjugated immunogen impact the quantity and 

 quality of B cell immune responses at both the gene expression 

 level and BCR sequence.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Roux2022.03.04.482938,

title = {The multi-level regulation of clownfish metamorphosis by thyroid hormones},

author = {Natacha Roux and Saori Miura and Mélanie Dussene and Yuki Tara and Fiona Lee and Simon Bernard and Mathieu Reynaud and Pauline Salis and Agneesh Barua and Abdelhay Boulahtouf and Patrick Balaguer and Karine Gauthier and David Lecchini and Yann Gibert and Laurence Besseau and Vincent Laudet},

url = {https://www.biorxiv.org/content/early/2022/03/04/2022.03.04.482938},

doi = {10.1101/2022.03.04.482938},

year  = {2022},

date = {2022-03-04},

urldate = {2022-01-01},

journal = {bioRxiv},

publisher = {Cold Spring Harbor Laboratory},

abstract = {Most marine organisms have a biphasic life cycle during which a pelagic larva is transformed into a radically different juvenile. In vertebrates the role of thyroid hormones (TH) in triggering this transition is well known, but how the morphological and physiological changes are integrated in a coherent way with the ecological transition remains poorly explored. To gain insight into this question, we performed an integrative analysis of metamorphosis of a marine teleost, the clownfish Amphiprion ocellaris. We reveal how TH coordinate a change in color vision as well as a major metabolic shift in energy production, hence highlighting its central integrative role in regulating this transformation. By manipulating the activity of LXR, a major regulator of metabolism, we also reveal a tight link between metabolic changes and metamorphosis progression. Strikingly, we observed that these regulations are at play in the wild revealing how hormones coordinate energy needs with available resources during life cycle.Competing Interest StatementThe authors have declared no competing interest.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}